In-hospital mortality was significantly higher in the high-XOR group (n = 28, 26.2%) than in the normal- (n = 11, 5.1%) and low- (n = 9, 8.3%) XOR groups. The high-XOR group (vs. normal-XOR group) was independently associated with the in-hospital mortality (OR 2.934; 95% CI 1.170-7.358; p = 0.022). Serum UA levels and plasma XOR activity were high in patients admitted to intensive care. The enhanced XOR activity may be one of the mechanisms under which hyperuricemia was associated with adverse outcomes in patients requiring cardiovascular intensive care.The aim of this study has been to evaluate the effect of sewage sludge and composted sewage sludge and municipal waste on the content of various forms of P in soil. The experiment scheme C, control; NPK; FYM; DGSS, dried and granulated sewage sludge; CSS, composed sewage sludge; CSSS, composted sewage sludge and straw; CMMW, composted mixed municipal waste; CMGW, composted municipal green waste. The content of bound P was determined in the fractions F1, easily soluble; F2, exchangeable; F3, organic; F4, carbonate; F5, stable organic-mineral and mineral bonds; and F6, residual. The NPK fertilisation as well as the soil fertilisation with organic substances raised the P-total content and of P bound in the fractions F3, F4, F5 and F6. The highest amount of phosphorus in the studied soil was in fraction F3 (phosphorus in organic compounds) and the lowest in fraction F1 (phosphorus in the ionic form as H2PO4- and HPO42-). selleck kinase inhibitor Composted sludge and straw introduced into the soil increased the content of readily soluble P (F1), while the NPK effect was reversed. NPK fertilisation and enhancement of soil organic matter (except CSSS, CMGW) led to a reduction of the P content in F2 fraction. The content of available P determined by the Egner-Riehm method depended on the content of C-organic, P-total and CEC soil. Among the determined phosphorus fractions, the content of available P was most strongly correlated with the content of P bound in the carbonate fraction (F4) and residual fraction (F6) and, less strongly, with the organic phosphorus fraction.A highly uniform and monodisperse silica-encapsulated Au@Ag multilayered core-shell nanorods (~ 80 nm in length) has been prepared with excellent electrocatalytic properties. Using the Au@Ag@SiO2 nanoassemblies to substantially enhance the sensitivity and the sol-gel molecularly imprinted polymer (MIP) with imprinted cavities to present special molecular recognition sites, a novel electrochemical sensing platform was rationally designed, fabricated, and tested for efficient theobromine (THB) quantification. The formation of final Au@Ag@SiO2@MIP was characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The performance of the Au@Ag@SiO2@MIP modified electrode was evaluated by differential pulse voltammetry with the changes in peak current of hexacyanoferrate redox probe measured at a working potential of 0.2 V (vs. saturated calomel electrode) as determination signal. Under optimal conditions, the quantitation of THB was attained in a broad linear range from 10 nM to 100 μM with a detection limit of 8.0 nM. The selectivity of Au@Ag@SiO2@MIP was examined according to its recognition to THB and the interferents. Finally, the sensing platform was successfully applied to extract and determine THB from food, biological, and environmental samples with acceptable recoveries (92.20-107.1%) and relative standard deviation less then  4%. The propsed sensor provides a robust means for monitoring alkaloids in complex matrices and a promising opportunity to develop sensitive and selective electrode materials with good reusability. Graphical abstract.PURPOSE The usual first-line strategy of wild-type EGFR (wtEGFR) non-small cell lung cancer (NSCLC) remains cisplatin-based chemotherapy. However, cisplatin often loses effectiveness because most tumors acquire drug resistance over time. As EGFR is the most important pro-survival/proliferation signal receptor in NSCLC cells, we aimed at investigating whether cisplatin resistance is related to EGFR activation and further evaluating the combined effects of cisplatin/gefitinib (EGFR-tyrosine kinase inhibitor, EGFR-TKI) on cisplatin-resistant wtEGFR NSCLC cells. MATERIALS AND METHODS EGFR activation was analysed in parental and cisplatin-resistant wtEGFR NSCLC cell lines (H358 and H358R, A549 and A549R). Cellular proliferation and apoptosis of H358R/A549R cells treated with cisplatin or gefitinib, alone or in combination were investigated, and the related effector protein was detected by western blot analysis. Anti-tumor effect of two drugs combined was evaluated in animal models of H358R xenografts in vivo. RESULTS EGFR was significantly phosphorylated in cisplatin-resistant wtEGFR NSCLC cells H358R and A549R than their parental cells. In H358R and A549R cells, anti-proliferative ability of gefitinib was further improved, and gefitinib combined with cisplatin enhanced inhibition of cellular survive/proliferation, and promotion of apoptosis in vitro. The combined effects were also associated with the inhibition of EGFR downstream effector proteins. Similarly, in vivo, gefitinib and cisplatin in combination significantly inhibited tumor growth of H358R xenografts. CONCLUSION Abnormal activation of EGFR may induce wtEGFR NSCLC cell resistance to cisplatin. The combined effects of cisplatin/gefitinib suggest that gefitinib, as a combination therapy for patients with cisplatin-resistant wtEGFR NSCLC should be considered.Nosocomial infections caused by COVID-19 will result in the immediate closure of the infected medical facility due to the highly contagious nature of COVID-19, further worsening medical shortages. We performed a Delphi consulting study to develop a risk model based on the experience of recently resumed activities in many cancer hospitals in China. Similar method had been used in a Chinese cancer hospital and effectively reduced the nosocomial Infection. We believe that the model can quickly identify COVID-19 cluster risks for medical facilities. The evaluation model could also be integrated into the hospital’s emergency response system in a timely manner.