Palladium has proven to be effective in catalyzing the (hetero)annulation of C[double bond, length as m-dash]C bonds with ambiphilic organo(pseudo)halides. Through the employment of appropriate ambiphilic coupling partners, efficient annulation of a variety of allenes, 1,3-dienes, strained alkenes, styrenes, and other C[double bond, length as m-dash]C bond variants can be achieved to provide direct access to numerous useful hetero- and carbocyclic scaffolds. In this Feature Article, we summarize palladium-catalyzed (hetero)annulation methods reported since 2005 (spanning just over 15 years) and discuss outstanding challenges in this area of study.Facile and simultaneous synthesis of diphenyl-disilabicyclo[14.14.14]alkane in/out-isomers was achieved by using organosilicon chemistry. Although the formation of several in/out-isomers would be conceivable, only two diastereomers, i.e. the (traditional-)out,out-isomer and the twist-out,out-isomer, could be isolated because of homeomorphic isomerization. Crystal structures of the diastereomers were confirmed.Tapping into the secondary coordination environment of mononuclear DyIII-complexes leads to drastic changes in luminescence and magnetism. Visualization of effects induced by stereoelectronics on the opto-magnetic properties was achieved through subtle modifications in the ligand framework.Since the first report by Yaghi’s group in 2005, research enthusiasm has been increasingly raised to synthesize diverse crystalline porous materials as -B-O-, -C-N-, -C-C-, and -C-O- linkage-based COFs. Recently, the biomedical applications of COFs have become more and more attractive in biomedical applications, including drug delivery, bioimaging, biosensing, antimicrobial, and therapeutic applications, as these materials bear well-defined crystalline porous structures and well-customized functionalities. However, the clinical translation of these research findings is challenging due to the formidable hindrances for in vivo use, such as low biocompatibility, poor selectivity, and long bio-persistence. Some attempts have raised a promising solution towards these obstacles by tailored engineering the functionalities of COFs. To speed up the clinical translations of COFs, a short review of principles and strategies to tune the physicochemical properties of COFs is timely and necessary. In this review, we summarized the biomedical utilities of COFs and discussed the related key physicochemical properties. To improve the performances of COFs in biomedical uses, we propose approaches for the tailored functionalization of COFs, including large-scale manufacture, standardization in nanomedicines, enhancing targeting efficacy, maintaining predesigned functions upon transformations, and manipulation of multifunctional COFs. We expect that this minireview strengthens the fundamental understandings of property-bioactivity relationships of COFs and provides insights for the rational design of their high-order reticular structures.Due to the ability to combine multiple osteogenic induction “cues” at the same time, hydrogels are widely used in the three-dimensional (3D) culture of human mesenchymal stem cells (hMSCs) and osteoinduction. However, the survival and proliferation of stem cells in a 3D culture system are limited, which reduces their osteogenic differentiation efficiency. In addition, the cells inside the hydrogel are prone to apoptosis due to hypoxia, which is a serious challenge for tissue engineering based on stem cells. In this study, a tripeptide-based macroporous alginate hydrogel was prepared to improve the osteogenic microenvironment of stem cells. The arginine-glycine-aspartate (RGD) peptide promoted the adhesion and proliferation of stem cells, and the degradation of gelatin microspheres (GMs) produced a macroporous structure to enhance further the migration and aggregation of stem cells. Mesoporous silica nanoparticles (MSNs) sustained-release bone-forming peptide-1 (BFP-1) induced osteogenic differentiation, and the sustained release of the QK peptide from the GMs promoted angiogenesis. In vitro experiments have shown that this functionalized hydrogel stimulates the proliferation of hMSCs, encourages larger cell cluster formation, and enhances the osteogenic differentiation efficiency. The released QK facilitates the proliferation and migration of endothelial cells. In vivo experiments have also verified that this system has a better osteogenic effect, and more blood vessels were observed inside the hydrogel, than in other systems. In general, this research has led to the development of a tripeptide macroporous hydrogel that can simultaneously promote osteogenesis and angiogenesis, showing great promise for applications of 3D cultures and stem cell-based tissue engineering.Pathogens are commonly present in the human respiratory tract, but symptoms are varied among individuals. The interactions between pathogens, commensal microorganisms and host immune systems are important in shaping the susceptibility, development and severity of respiratory diseases. Compared to the extensive studies on the human microbiota, few studies reported the association between indoor microbiome exposure and respiratory infections. In this study, 308 students from 21 classrooms were randomly selected to survey the occurrence of respiratory infections in junior high schools of Johor Bahru, Malaysia. Vacuum dust was collected from the floor, chairs and desks of these classrooms, and high-throughput amplicon sequencing (16S rRNA and ITS) and quantitative PCR were conducted to characterize the absolute concentration of the indoor microorganisms. Fifteen bacterial genera in the classes Actinobacteria, Alphaproteobacteria, and Cyanobacteria were protectively associated with respiratory infections (p less interactions among these factors.A turn-on optoacoustic and NIR-II fluorescent probe for imaging antituberculotic drug-induced liver injury has been developed. Epigenetic Reader Domain inhibitor Probe TC-H2O2 responds to hepatic H2O2, thus releasing chromophore TC-NN, which displays prominent NIR-II fluorescence and optoacoustic signals for diagnosing liver injury.