Blood vessels serve as a versatile transport system and play crucial roles in organ development, regeneration, and stem cell behavior. In the skeletal system, certain capillaries support perivascular stem cells or osteoprogenitor cells and thereby regulate bone formation. Recent studies reported that a specialized capillary subtype, termed type H vessels, is shown to couple angiogenesis and osteogenesis in rodents and humans. They can be distinguished by specific cell surface markers, as the endothelial cells in the metaphysis and endosteum highly express the junctional protein CD31 and the sialoglycoprotein endomucin. Here, we provide an overview of the role of type H vessels in bone homeostasis and summarize their linkage with various cytokines to control bone cell behavior and bone formation. We also discuss the potential clinical application for bone disorders by targeting these specific vessels according to their physiological and pathobiological settings. © 2020 New York Academy of Sciences.Neuronopathic Gaucher disease (nGD) has a very wide clinical and genotypic spectrum. However, there is no consensus definition of nGD, including no description of how best to diagnostically separate the acute form-Gaucher type 2-from the subacute or chronic form-Gaucher type 3. In this article, we define the various forms of Gaucher disease with particular emphasis on the presence of gaze palsy in all patients with nGD. This consensus definition will help in both clinical diagnosis and appropriate patient recruitment to upcoming clinical trials. © 2020 The Authors. Journal of Inherited Metabolic Disease published by John Wiley & Sons Ltd on behalf of SSIEM.Exposure to phosphine (PH3 ), a common grain fumigant, is characterized by diverse nonspecific symptoms and a high mortality rate. Although PH3 poisoning is thought to target oxidative respiration, the exact mechanism of action remains largely unknown, resulting in limited treatment options. In our study, the effects of PH3 on female rats were assessed to elucidate potential sex-specific differences and obtain a more comprehensive understanding of PH3 toxicity. Lethality, physiology, and behavior were evaluated in female rats exposed to gaseous PH3 (13,200-26,400 ppm × min), and results were compared with corresponding findings in male rats. Median lethal concentration-time (LCt50 ) and time of death (tTOD ) did not differ significantly between the sexes. Cardiopulmonary changes induced by PH3 were also of comparable magnitude, although temporally, respiratory responses occurred earlier and cardiovascular variations manifested later in female rats. Behavioral observations corroborated physiological findings and indicated a response to hypoxic conditions and low cardiac output. Together, these results provided insights on the toxic mechanisms of PH3 , in particular, its potential interference with oxygen transport and circulation. © Published 2020. This article is a U.S. Government work and is in the public domain in the USA.BACKGROUND Efficacy of alleviating signs/symptoms of malignant ascites by a renovated CART (cell-free and concentrated ascites reinfusion therapy) system, called KM-CART, was evaluated. METHODS A total of 4781 KM-CART procedures was performed in 2109 patients. All patients were accepted unless hemodynamically unstable or consciousness impaired. The ascites were processed and drip-infused into the patient. RESULTS There were no major complications or deaths. The mean drainage volume was 6.2 L (maximum 27.7L), patient symptoms (numerical scale system) were significantly alleviated (45.1 ± 19.0 reduced to 21.2 ± 14.2, P less then 0.001), and patient leg circumference significantly decreased (33.3 ± 4.4 cm reduced to 30.5 ± 4.4 cm, P less then 0.001) without exacerbation of renal function. Collected cancer cells could be utilized for immune therapy. CONCLUSIONS KM-CART is capable of improving the “quality of best supportive care” and can be beneficial in conjunction with medication for malignant pain. BGJ398 order This article is protected by copyright. All rights reserved.Biodiversity-ecosystem functioning experiments found that productivity generally increases with species richness, but less is known about effects of within-species genetic richness and potential interactions between the two. While functional differences between species can explain species richness effects, empirical evidence regarding functional differences between genotypes within species and potential consequences for productivity is largely lacking. We therefore measured within- and among-species variation in functional traits and growth and determined stand-level tree biomass in a large forest experiment factorially manipulating species and genetic richness in subtropical China. Within-species variation across genetic seed families, in addition to variation across species, explained a substantial amount of trait variation. Furthermore, trait responses to species and genetic richness varied significantly within and between species. Multivariate trait variation was larger among individuals from species mixtures than from species monocultures, but similar among individuals from genetically diverse vs. genetically uniform monocultures. Correspondingly, species but not genetic richness had a positive effect on stand-level tree biomass. We argue that identifying functional diversity within and among species in forest communities is necessary to separate effects of species and genetic diversity on tree growth and community productivity. This article is protected by copyright. All rights reserved.A simple and effective ratiometric fluorescent sensor of CdTe QDs/GCNNs for on-site and rapid analysis of Cu2+ has been established by mixing physically CdTe QDs and graphite carbon nitride (GCNNs). Two emissions peaks of CdTe QDs at 572 nm and GCNNs at 436 nm are both excitated at 340 nm. Under a UV lamp, fluorescent of traffic yellow CdTe QDs is linearly quenched by Cu2+ (as the detection signal), while blue GCNNs remains unchanged (as the reference), resulting in a distinguishable color change gradually from pink yellow to blue. The limit of detection (LOD) of this new sensor for Cu2+ is as low as 0.47 ng mL-1 with 1.4 % RSD. The established method has been successfully applied to detection of Cu2+ in various drinks with satisfactory results. Moreover, a paper-based sensor, which has been prepared by soaking cellulose acetate membrane in CdTe QDs/GCNNs sensor solution, has a wide semi-quantitative detection range for Cu2+ (0.01~5.0 μg mL-1 ). It has realized successfully on-site and rapid determination of Cu2+ in red wine without any pretreatment procedure and is of great promotion and application value in determination of Cu2+ in liquid samples.